In this study, quantitive characteristics and chemical composition of in roach (Rutilus rutilus caspicus) sperm were investigated. Sperm traits included sperm movement duration, percentage of motile spermatozoa, sperm density, spermatocrit and sperm volume. Some seminal plasma characteristics (sodium, potassium, calcium, magnesium and chloride) were investigated. In addition, some metabolites of seminal plasma (glucose, cholesterol and protein) were measured. The Na+ and K+ ions correlated negatively with spermatozoa motility (r= -0.0518, p<.05 and r=-0.3597, p<.01) respectively. Also, there were significant positive correlations between Ca2+ and Cl- ions with spermatozoa motility (r= 0.2945, p<.05 and r= 0.1379, p<.01), respectively. Mg+2 was positively correlated with glucose and protein (r= 0.046, p<.05 and r= 0.694, p<.05), respectively. On the other hand, a significant positive relationship was found between Na+ and K+ (r= 0.548, p<.01). These parameters can be used to evaluation of sperm quality and collecting information about developing procedures for artificial fertilization of roach.