Benthic invertebrates are a well-established target in environmental quality status evaluations. The AMBI (AZTI's Marine Biotic Index) was developed to determine the impacts and the quality status of soft-bottom marine benthic communities. The aim of this study was investigating multivariate pattern of benthic invertebrate community and application of AMBI (AZTI's Marine Biotic Index) for determining quality of health status in Khuzestan coastal waters. Seasonal samplings were collected from eight creeks over one year study by Peterson grab sampler, beginning from October 2005. All creeks are characterized by muddy bottom. According to sensitivity to environmental stress, macrobenthic animals classified into 5 ecological groups. Among 28 identified faunal groups the most abundant groups were polychaets, molluscs, crustaceans and nematodes, respectively. Two way ANOVAs showed only seasonal significant differences in mean abundance (P<0.05) and Margalef richness index values (P<0.05), while all of studied creeks shows similar biological characters based on benthic communities. According to annual mean of AMBI value all creeks were in ecological group III with slightly polluted condition, except for Darvish that was in unpolluted category. In the present study, although the number of species initially increased, but due to appearance of dominant species such as Capitella sp. and nematods (as opportunist species), diversity values reduced. In general, according to AMBI and Biotic index values, the most creeks are classified into unpolluted and slightly polluted categories except for Zangy, Doragh and Patil in summer and also Zangy and Bihad in winter which was in moderate to heavily polluted category. Different types of exploitation, industrial and shipping activities in this area could result in unbalanced to polluted status in benthic animals (as the best indicators in sediment quality assessment) so these results can be acceptable.