This study was conducted to investigate the effects ofCurcuma zedoariaandZingiber zerumbet on non-specific immune responses of grouper (Epinephelus coioides). Fish were fed an experimental diet containing 0, 0.5, 1.0, 2.5 g/kg ofC. zedoariaandZ. zerumbetmixed diets twice daily for two weeks. Non-specific immune parameters such as respiratory burst activity, reactive oxygen species, phagocytic activities, superoxidase dismutase activity and lysozyme activity were sampled at 0, 1, 2, 4, 7 and 14 days, respectively. Results indicated that in fish fed withC. zedoaria at 0.5 g/kg diet andZ. zerumbetat 1 g/kg and 2.5 g/kg diets the non-specific immune response was affected, especially in cellular defense which had significant effects in the short term. Thus, this study indicated thatC. zedoaria andZ. zerumbetsupplemented in the diets of orange-spotted grouper acted as immunostimulants and appeared to enhance the non-specific immune responses in this species.

Keywords:Herbs, Immunology, Phagocytosis, Plant extract, White blood cell