The present study investigated the use of fermentedProsopis juliflora pods (PJP) as a carbohydrate source alternative to rice bran (RB), and aP. juliflora pods based diet formulation supplemented with probioticsLactobacillus acidophilus (LA), Lactobacillus bulgaricus 009 (LB) andStreptococcus thermophilus (ST) on the growth ofLabeorohita (rohu) fingerlings. The diets were isonitrogenous (39%) and isoenergetic (15.9 KJg-1 gross energy); and were prepared using rice bran (as reference diet RD), crude pods (PJPC), fermented pods (PJPF) and with probioticsL. acidophilus (PJPLA),L. bulgaricus (PJPLB) andS. thermophilus (PJPST). The results showed that fermentation ofP. juliflora pods withL. acidophilus significantly increased (p<0.05) protein, lipid, Ca, Fe, Mg, K and reducing sugar; and completely eliminated phytic acid. The diets PJPF and PJPLA produced significantly higher (p<0.05) percent weight gain, SGR, FCR and PER as compared to PJPC, PJPLB and PJPST diets; and achieved growth performance comparable to RD. An improvement (p<0.05) in the carcass protein has been detected in groups PJPF and PJPLA in comparison to RD, PJPC, PJPLB and PJPST. TheL. acidophilus was found to be effective in controlling coliform count in gut as compared toL. bulgaricus andS. thermophilus; and also in controlling total viable count, total coliform count and fecal streptococci count in fecal matter. The results indicate that PJPF and PJPLA deserve further investigations as alternative feed for rohu.